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The purpose of this study is to synthesize methyl esters from copper oil (Voor-Oogst) with KOH catalyst using ultrasonic waves and potential test as biodiesel. Tobacco seed samples were obtained from the Paiton Probolinggo . The stages in this study are: isolation of tobacco seed oil using soxhletation with n-hexane solvents; decreasing free fatty acids by esterification process; transesterification with variations in KOH catalyst concentration; characterization of synthesized biodiesel; and identification of components of methyl ester synthesis by GC-MS. The results showed: Synthesis of methyl esters with transesterification reaction at 0.8% KOH catalyst concentration; 1% and 2% w / w of oil with ultrasonic waves produce biodiesel with the result are 77.45% respectively; 84.55% and 80.72%, (2) Methyl esters from tobacco oil fit the SNI biodiesel criteria with a viscosity of 4.44 cSt, density 0.865 g / mL, refractive index 1.45, acid number 0.56 mg/g. The main constituent of methyl ester which is synthesized in the form of methyl palmitate, linoleic, oleic and stearic is 18.72% respectively; 30.76%; 27.26% and 9.35%.