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One of the compounds contained in neem is flavonoid glycosides, which have anti-fertility effects by reducing hyaluronidase activity, so can prevent penetration of spermatozoa in mice. The mechanism of inhibition of spermatozoa penetration by flavonoids caused by decrease in hyaluronidase activity possible of the influence of gene expression disturbances, so enzyme synthesis decreases. However, the mineral contained in the seeds and leaves of neem can induce oxidative stress in animals, characterized by the increase of Lipid Peroxidation Potential (LPP) in tissues. LPP can be determined by measuring malondialdehyde (MDA) molecules taking the standard thiobarbituric acid (TBA) test. Male mice were divided into nine groups: control (C0), Group t1 - t4 were treated by administering water seeds and leaves extract of neem at doses 0.25 and 0.50 mg/kg bw for 36 days through intraperitoneal injection, without reversibility testing, group T5-T8 were treated by administering water seeds and leaves extract of neem at doses 0.25 and 0.50 mg/kg bw for 36 days followed by reversibility testing for 36 days, with four replications. The results showed the administration of water seed and leaves extract of neem could reduce the MDA levels of mice testis. Conclusion is quality of testicular mice is influenced by oxidative stress conditions in male mice.