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Ambon Bay is located on Ambon Island, which most of the area is within the Ambon City, the capital of Maluku Province. Coastal ecosystems such as coral reefs, mangroves, and seagrass beds still exist on this bay. However, as a coastal ecosystem in a crowded city, the potential of degradation for these ecosystems is very large. Over the past five years, many changes have taken place in this bay. In the land section, the construction of housing and large buildings such as shopping centers has occurred. In addition, on the coast, there has been a construction of new docks and several locations have been reclaimed. Therefore, in 2016 there was a re-observation of the seagrass conditions at all transect locations of 2011, to see the changes that have occurred in seagrass beds in Ambon Bay over the past five years. The results will be compared to the satellite imagery of each location. Overall, the condition of seagrass beds in Ambon Bay in 2016 has decreased from the initial observation in 2011. Of the seven research locations, only one location (Halong) has an increase in its conditions, with seagrass canopy cover rise by almost twofold from previous data (24.29% rise to 50.00%). Four locations experienced a decline in conditions, from a slight decrease in Waiheru and Lateri (25.00% down to 20.91% and 47.14% down to 30.00% respectively) to a sharp decrease in Tanjung Tiram and Passo (65.67% down to 39.33% and 48.33% down to 16.25% respectively). The remaining two locations (Tantui and Hative) were in worse condition because they were damaged by the reclamation project. From 2011 and 2016 satellite image comparison, almost all locations show changes in their land use, with more building built near and on the coastal area, except in Halong. This indicates that seagrass condition negatively affected by coastal development.