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Chrisan flower is one of decorated flower with hight quality to cultivated by Indonesian citizen. However, but in cultivation process had one of problem is Karat Putih disease coused by Puccinia horiana Henn. Using biofungicide from betel leaf extract (Piper betle Linn.) one of solution for this problem. The purpose from this research is know effectiveness from betel leaf extract as biofungicide to solve Karat Putih disease and know consentration of betel leaf to impede growth fungus Puccinia horiana Henn. This reaseach have been 7 month, begun May-November 2018. This research is experimental which using Random Plans Complex (RAL) Method with 5 repititions. The treatment be differentiate as many concentration of betel leaf, like: 0%, 30%, 40%, 50%, 60%, and 100%. The parameter of this research is measuring diameter of Karat Putih fungus everyday until 5 days. The result indicate the 40% concentration of betel leaf is most effective to impeded growth fungus Puccinia horiana Henn until diameter of growth only 0,3% (the most impeded).