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Silver material has been successfully synthesized using the chemical reduction method, while Mn0.25Fe2.75O4/silver material has been successfully fabricated using the coprecipitation method. The characterization of the crystal nanostructure of the material was carried out using an XRD instrument whose results showed the appearance of diffraction peaks corresponding to the magnetite and silver phases. The characterization of functional groups from the sample was carried out using FTIR instruments whose results showed that the functional group bonds produced corresponded to the wavelength range of the material constituents of Mn0.25Fe2.75O4/silver. The magnetic properties of Mn0.25Fe2.75O4/silver material were successfully characterized using VSM instruments. The results obtained are in the form of a hysteresis curve which shows that the sample Mn0.25Fe2.75O4/silver has superparamagnetic properties which are indicated by a coercive field value that is close to zero. While the antimicrobial activity was characterized by using the diffusion method and the results showed that the anti-microbial activity of the sample /silver worked very well. This result is characterized by the emergence of microbial free zones around the sample Mn0.25Fe2.75O4/silver with a significant radius.