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Antibiotic resistance is currently an emerging global threat that is triggered by misused and overused of antibiotics, which led to higher mortality and morbidity rate. Information of antibiotic resistance iscrucial to determine the ideal treatment for patients. Since these data is rather scarce in Bali, we performed antibiotic susceptibility profile of different clinical bacterial isolates in the General Hospital Sanjiwani Gianyar Bali based on retrospective data in 2018. The data included type of tested antibiotics, name of clinical bacterial isolates, sex of patient and antibiotic resistance profile. Among 65 clinical bacterial isolates, high diversity of bacterial pathogen was found and grouped into 22 species, which Escherichia coli, Klebsiela pneumoniae and Staphylococcus aureus were the three predominant isolates identified. Exposure against 28 different antibiotics showed majority of bacterial isolates were resistant against baxtrim, aztreonam, ciprofloxacin, but susceptibility towards meropenem, amikacin and cefoperazone-sulbactam were observed. Routine surveillance to determine antibiotic resistance profile are of important and recommended to generate a local antibiogram as guideline to overcome pathogenic infections.