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Abstract. The Depo medroxyprogesterone acetate (DMPA) is a type of hormonal contraceptive containing progesterone that causes a hypoestrogenic response that inhibits follicle development, prevents ovulation, and causes endometrial atrophy due to suppression of epithelial and stromal cell proliferation. Natural herbs like bengkoang (Pachyrhizus erosus) can be used to reduce the negative effects of DMPA. Bengkoang is widely known as containing isoflavones with chemical properties and functional structures that resemble 17eta estradiol. This study aimed to determine the effect of bengkoang ethanol extract on the number of ovarian follicles, epithelium, and endometrial stroma in DMPA treated Rattus norvegicus. This study used 25 rats as experimental animals, which were divided into five groups consisting of one negative control group (KN) without DMPA and bengkoang extract, and four treatment groups given DMPA at 2.7 mg every three days repeated four times. After the induction period, the hypoestrogenic condition was evaluated through vaginal smear. Three groups were treated by bengkoang extract at a dose of 70 mg/200g BW/day (P1), 140 mg/200 g BW/day (P2), and 280 mg/200 g BW/day (P3). In this study, data were analyzed using a one-way ANOVA and the Least Significant Difference (LSD) test. The results showed that there were significant differences (p< 0.05) in the number of antral follicles, the amount of the epithelium, and the amount of endometrial stroma in the group treated by bengkoang extract dose of 280 mg/200g BW/day. This study suggests that bengkoang extract demonstrated a change in the number of ovarian follicles, epithelium, and endometrial stroma compared to the positive control group treated only with DMPA.