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Genetically, each individual in one variety of oil palm is varies, both from the level of relationship and the level of diversity. Genetic diversity can be determined used one of the molecular markers, that is RAPD. The study aims to determine genetic diversity of oil palm (Elaeis guineensis Jacq.) DxP 540 NG variety which is moderate variety of resistant ganoderma based using 3 RAPD markers. The study is conducted at the Plant Biotechnology Laboratory, Faculty of Agriculture, University of Sumatera Utara, Medan. The genetic materials analyzed came from 20 of oil palm D x P 540 NG variety. Genetic bands size measured using UVITEC Cambridge FireReader, genetic distances calculations obtained Principal Coordinates Analysis (PcoA) and Neighbor-Joining Tree (NJTree) analysis using software DARwin 6.0. The results showed that the 20 plants had genetic distances ranging from 0 - 0.60 and were divided into 3 main groups with unquilified molecular diversity of 54.31%.