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Background: Hemodialysis is an effective treatment carried out for patients with chronic kidney failure as an effort to improve the quality of life. 80% of hemodialysis patients experience physical stress disorder and psychosocial stress related to treatment. When faced with stress, the muscles of the body become tense as the abdomen becomes tense and cramped, the chest feels tight which has an impact on physical and psychosocial health so it is necessary to have stress management training through relaxation techniques. The purpose of this study was to determine the effect of progressive relaxation on stress levels in hemodialysis patients. Method: This is a quasi-experimental study with a pre-test and post-test with control group design. There were 58 respondents divided into 2 groups (intervention and control) and each group consisted of 29 people selected by random sampling. Measurement of stress levels using questionnaire Depression Anxiety Stress Scale 42 (DASS 42). Results: The Mann Whitney U Test showed a difference in the decrease in stress levels which was statistically significant (p <0.05). Conclusion: The audiovisual progressive relaxation training conducted at least 2 times a week for 3 weeks is effective for reducing stress levels in patients undergoing hemodialysis therapy for at least 1 month