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This study was conducted to determine the effect of Ipomoea batatas L. leaf extract as flocculants on the reduction of Ca2 + and Mg2 + (hardness) levels in water treatment for chemical practicum on environmental analysis at the FPMIPA UPI Chemical Department. This study continued from previous research on the optimization of Ipomoea batatas L. leaf extract as a flocculant in water treatment, the results of the study showed that Ipomoea batatas L. leaf extract can make water clearer and provide greater reduction in turbidity value. In this follow-up study it will be carried out through two stages, the first step is optimizing the optimum processing parameters. The second stage is water treatment using optimum processing parameters, followed by hardness testing in water samples before and after processing. In hardness testing the EDTA titration method is used with the EBT indicator. The results showed the optimum parameters of water treatment with Ipomoea batatas L leaf extract as flocculant was pH 6.5, coagulant dose 50 mg / L, flocculant dose 2 mg / L, stirring coagulant speed 200 rpm for 5 minutes, and flocculant stirring speed 40 rpm for 5 minutes. In testing the total hardness as CaCO3, the initial sample (without treatment) obtained hardness of 285 mg / L, whereas in the sample treated with Ipomoea batatas L. leaf extract as flocculant hardness was obtained at 190 mg / L. From the results of the study it can be concluded that Ipomoea batatas L. leaf extract as flocculant in water treatment can reduce the hardness level in water.