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Embryogenesis of microspore or androgenesis is one of method that extensively uses in breeding programs to produce embryos and plantlets with the number of haploid or double haploid chromosomes with homozygous or pure lines in a relatively short time. Immature male gametophytes can change their developmental fate from pollen to embryo when exposed to stress treatment during in vitro culture. Stage development of microspore is one of the important factors that determine the success rate of induction of microspore embryogenesis. In this study, stage development of microspore of eggplant cultivar Planet Hijau was determined through morphological analysis of microspore cells based on the morphological size of flower buds to optimize the induction of embryogenesis. The results showed that the interval of flower buds 15-18 mm and the interval of petal length 7-10 mm contained 50-55% late uninucleate microspores. In determining the stage of development of microspores, the length of anther and diameter of anther are also considered. The flower bud of eggplant which contain late uninucleate and binucleate microspore is the best stage to induction of embryogenesis.