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The aim of this study was to determine the level of adoption of a range of standard biosecurity practices in beef cattle farmers in Soppeng Regency, South Sulawesi. Data were collected from 45 beef cattle farmers through observation and in-depth interview by using questionnaire. There was 26 questions which consisted of biosecurity practices: sanitation, isolation and traffic control. Data were analyzed descriptively using mean and percentage. The results showed that a level of partial adoption of biosecurity had been achieved by the beef cattle farmers. The implication is that beef cattle farmers could be motivated to enhance their level of biosecurity practices.