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Myoglobin has an important role in maintaining the oxygen homeostasis within living tissues. Therefore, the existence of myoglobin is very important to be studied in hypoxia tolerant animals, in this case the Brazilian turtle (Trachemysscriptaelegans). This study aims to detect myoglobin expression in the heart, kidney and lung of Brazilian turtle using the myoglobin primers Myoturtle_F2 and Myoturtle_R1. This study was performed using the RNA total of the heart, kidney, and lung of Brazilian turtles, and using various primer concentrations and annealing temperatures. This study was conducted at the Laboratory of Biochemistry and Molecular Biology of FMIPA UNJ from June to August of 2010. Through 2% agarose gel electrophoresis, a myoglobin band of 114 pb had been detected in the kidney of normoxic Brazilian turtle. In the lung of the Brazilian turtle, myoglobin was not detected which might be caused by the low myoglobin concentration within the organ.