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‘Pedetan- is one of the food products of the traditional Balinese fish sardine which is processed by the community in the Jembrana Regency of Bali Province. This study aims to determine the antimicrobial activity of garlic and kaempferia galangal in inhibiting the growth of Aspergillus sp. which can contaminate the sardine fish Pedetan, The study was conducted by isolating Aspergillus sp. from Pedetan and testing the inhibitory power of garlic and Kaempferia galangal The results showed that the extract of garlic and Kaempferia galangal can inhibit the growth of Aspergillus sp. From the results of SEM observations it was found that garlic and kaemfiera were able to damage the Aspergillus sp. Mycelia. compared to control treatment. Garlic extract and kaempferia galangal can inhibit the growth of Aspergillus sp. because they contains bioactive compounds that are antimicrobial.