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This study conducted to characterization amino acids of the antioxidant bioactive peptides from Indonesian local meats among them P.O beef, Kacang goat meat, Mojosari duck meat and local chicken meat. The research was conducted in the Lamongan district of East Java. The method was laboratory exploration. The variables observed included antioxidant activity, amino acids profile with LC-MS/MS. The results of this study indicate variation in antioxidant activity of various local Meats in Indonesia. The amino acids profile also exhibit diversity with each other. Amino acids obtained are distributed evenly to the types of essential and non essential amino acids.