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Today the communitys needs for rehabilitation materials are quite large, so it needs to be developed to find alternative rehabilitation materials that are good, affordable to the community and capable of replacing lost network structures without causing negative effects. The purpose of this study was to determine the characterization of hydroxyapatite biomaterials from Java sea cuttlefish (Kendal) by XRD testing to be compared with HAp 200 Teihei from Japan. The material for making hydroxyapatite [Ca10 (PO4) 6 (OH) 2] is using two types of cuttlefish (squid and cuttlefish) from the Java Sea (Kendal) which are reacted with a solution of diammonium hydrogen phosphate [(NH4) 2HPO4] in a closed space (pressure vessel) by hydrothermal process using a microwave at a temperature of 200oC for 4 hours. Then to find out the results of the characterization of the test material, the XRD (X-Ray Diffractometer) machine was tested which further indicated that there was similarity with commercially available synthetic hydroxyapatite (HAp 200 Teihei from Japan) as a comparison material. From the analysis of XRD testing that has been done for variations of the type of cuttlefish used, it was found that the peak-peak pattern possessed by CT HAp from cuttlefish is far more dominant to become hydroxyapatite than CT HAp from squid. Meanwhile the CT characterization of HAp from cuttlefish does not completely change to hydroxyapatite, but there is a change to become hydroxyapatite. This is shown by the formation of peak-peak patterns from cuttlefish CT HAp at the peak of 31.801o, 32.178o, and 32.94o which are almost similar to the Teihei HAp 200 from Japan.