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Aspergillus sp. mold is found in various habitats, especially on the ground. Land waste disposal is a good medium for the growth of microorganisms due to the condition of the land that is rich in organic matter. This study is expected to provide information about the potential of Aspergillus sp. mold mainly from soil which can produce antimicrobial compounds. This study aims to determine whether the metabolic metabolites of Aspergillus sp. obtained have activities as antimicrobials against Staphylococcus aureus, Salmonella thyphosa and Candida albicans. The fermentation was carried out with a static fermentation method which was incubated for 5 days and the antimicrobial activity test was carried out by the diffusion method to use cylinders with 3 replications. The results of macroscopic and microscopic isolation and identification obtained five isolates from genera of Aspergillus sp. which were coded N1, N2, N3, N4 and N5. The metabolites were obtained from the fermentation of each fungus isolate Aspergillus sp. The test results of the metabolite activity of fungus Aspergillus sp. had antibacterial activity, against Staphylococcus aureus, Salmonella thyphosa and Candida albicans.