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Studies on mechanical and barrier properties of Cassava starch/PVA composites reinforced with pineapple leaf nanocellulose have been done to obtain an alternative new biodegradable food packaging. The aim of this research is to investigate changes of mechanical and barrier properties of cassava starch/PVA/Nanocellulose compared with cassava starch/PVA composite. Composition of Starch/PVA was 50:50 with nanocellulose concentration vary from 1, 3, 6 and 10% in bionanocomposites. Tensile strength, elongation, and water vapor transmission rate were used to evaluate the mechanical and barrier of bionanocomposite films. The microstructure was evaluated using FT-IR, SEM, and XRD. Results showed that increasing the concentration of nanocellulose decreasing the tensile strength and elongation of bionanocomposites. Nanocellulose decreased the transparency of the composite films. Addition of 1% nanocellulose increased the WVTR and then the WVTR of bionanocomposite films containing 3 – 10% nanocellulose decreased up to 10 – 20%.