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The purpose of this study was to analyze the validity and reliability of science virtual test to assess junior high school students- science process skills on levels of organization and cell transport topics, SVT-LOCT. The final design of the instrument was administered to 118 students of 7th graders from two schools in Kabupaten Bandung, Indonesia. The three steps to validate SVY-LOCT are content analysis of levels of organization and cell transport topics, expert judgment and readability test, and trial test (small group try out and larger test). SVT-LOCT was judged through education, content, media, and language by experts and readability test was conducted by teachers and students. The validation resulted in 31 test items that represent eight skills of SPS to assess students- science process skills in levels of organization and cell transport topic. The reliability of SVT-LOCT was considered as high with Cronbach alpha = 0.753 with correlation of XY = 0.65, meaning that the instrument is sufficient to assess students- science process skills.