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ABSTRAK The growth of microorganisms in the media is influenced by physical factors and chemical factors. Growth media must contain nutrients such as proteins and carbohydrates. Nuts, have a source of protein for the growth of fungi. Reshuffle bean material into simpler compounds through extrasi and germination are compounds that are good for the growth of fungi. The aim of research to determine the effectiveness of media Extraction And Soybean Sprouts Against fungus growth, and determine an effective medium. An experimental study by measuring the diameter of fungal growth for 5 days at a concentration of 2, 3, 4, 5 and 6% media seed extract and soy bean sprouts. The implementation was carried out at the Yogyakarta Health Analyst Health Laboratory, from May to September 2018. Subjects were Candida albicans, Sacharomyces cerevicea, Rhizopus Oligophorus and Aspergillus flavus, 24 hours old. The research object of local soybeans is of good quality, whole seeds, not rancid, not grained. Results, 5% soybean extraction medium was effective for the growth of Rhizopus oligosporus filamentous fungi, and 4% concentration was effective for the growth of fungi Aspergillus flavus. Media soya bean sprouts 3% effective concentration for pertumbhan fungi colonize namely Saccharomyces sereviceae, and concentration 6%, effective for the growth of the fungus Candida albicans. In conclusion, the extraction medium and soybean sprouts can be used as a substitute for the median SDA for fungi growth