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The laying hens livestock industry is growing rapidly along with the increasing demand for eggs for human consumption, will produce large amounts of waste. Improper management of laying chicken farm waste can interfere with health and environmental pollution including greenhouse gases (CH4, CO2, N2O), odor disorders, disturbances from rodent animals, disturbances of endoparasites and ectoparasites, pollution of water and soil sources. Appropriate waste management can reduce the risk of pollution of the laying hens industry to the environment. Utilizing feces of laying hens as a microbial consortium sources that serves as a biogas starter in anaerobic digester as an alternative environmental friendly energy source is an option. The purpose of this study was to obtain a bacterial and methanogen consortium from laying hens feces as a starter of biogas with coal media in anaerobic digester. The study used an experimental method of completely randomized design (CRD) with 4 doses and 4 replicates with 5 observations, data than tested further using orthogonal polynomials. The stages of the study included three stages: first, pretreatmen using in vitro technique; second, the adaptation process; third, addition starter of microbial consortium from the laying feces of the chicken into liquid media and coal at a dose of 0%, 5%, 10% and 15% then incubated at 39oC for 28 day. Observations were conducted every 7 days from day 0, day 7, day 14, day 21 and day 28. The parameters measured were the volume of biogas, the number of anaerobic bacteria and the composition of biogas. This biogas composition was analyzed by Gas Chromatography, the number of anaerobic bacteria cultured in Hungate tubes and calculated using the Ogimoto method. The observations showed that the number of bacteria ranging from 1012 CFU/ml up to 1013 CFU / ml exceeded the starter requirements of 107 CFU/ml.