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Fatty Acid Sorbitol Ester (FASE) is sorbitol ester from fatty acid with esterification degree point ranges from one to six. FASE with esterification degree above three can be used as fat replacer and below three can be used as surfactant. The aim of this study are to determine optimum conditions which include temperature, time, and concentration of papaya latex lipase enzyme, sorbitol, Fatty Acid Methyl Ester (FAME) in the synthesis of FASE as biosurfactant and to know the characteristics of FASE. This research consists of three phases, that are preparation of papaya resin and making of FAME, determination the optimum conditions (temperature, time, and enzyme concentration) of FASE synthesis by using Response Surface Methodology (RSM). The last phase is characterization of FASE. FASE synthesizes conducted by sorbitol esterification with palmitat-stearat FAME by using papaya lipase as catalyst. The result shows that the optimum condition of FASE synthesis at 41,02C, 52,35 minutes, with papaya lipase concentration of 10,30 percent and sorbitol conversion into FASE 31,43 percent. Biosurfactant FASE has melting point 27-30C, smoke point 140C, refraction index 1.44, and HLB value 7.5. 1percent FASE can hold emulsion of water in oil for 92 minutes.