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The use of plant roots as traditional medicine for Dayak Desa tribe is carried out from generation to generation but has not been recorded properly. This study aims to identify the types of plants used, parts of plants used, and the way they are processed by Dayak Desa tribe, from Kayan Hilir District, Sintang Regency, Indonesia. The survey research was conducted in March 2018 by seeking information from the community through interview and field observation. Information was obtained through semi-structured interviews with 100 informants regarding the types of plants used, the local names, the parts used, and the way to process them. Data analysis was done by compiling and classifying medicinal plants based on its family, its benefits, and its way of using. Then the data were calculated in the form of a percentage of families and the percentage of the way to use them. The results showed that twenty-five medicinal plants were used in twenty-two families. The family that was widely used were families of Araceae, Fabaceae, and Poaceae with a percentage of 8% each. The part of the plant used by Dayak Desatribe was the root that was used by way of boiling and drinking with a percentage of 60%.