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Banana peels waste contains lignocellulose which can be converted into starch and then broken down into glucose which will produce bioethanol. This study aims to reduce organic waste and determine the enzymatic activity of Aspergillus fumigatus fungi on banana skin enzymatic hydrolysis in the manufacture of bioethanol. The process starts from delignification followed by the hydrolysis process using a chemical catalyst, H₂SO₄ as a comparison and biological catalyst namely Aspergillus fumigatus. The study showed that the hydrolysis process was successfully carried out using a biological catalyst at a temperature of 30°C and a contact time of 3 days. During the hydrolysis process, the starch content was 3% - 4% using the DNS method. This shows that the use of banana peels waste containing lignocellulose can be used as a source of bioethanol which aims to reduce organic waste.