Indonesia Conference Directory

Wireless magnetoelastic (ME) biosensor was designed to detect one of the most popular foodborne pathogen, Escherichia coli (E. coli) O157H7. Binding of the bacteria to the antibody on the gold-coated ME sensor surface changed the resonance frequency of the sensor under the magnetic field, which was quantified by a network analyzer. The response of the sensor to the increment of the concentration of E. coli was investigated. A detection limit of 4.5x105 CFU/mL with a sensitivity of 4.7 kHz were achieved by the sensor with the dimension of 1x0.2x0.023 mm3. The immobilization of antibody-antigen was proven with the fluorescence microscopy which measured the fluorescence dye at the antibody to confirm the successful performance of the immune response on the sensor surface. The results demonstrate that the wireless ME biosensor has good potential to detect E.coli rapidly and efficiently.