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Java long pepper is a plant has an aphrodisiac effect. Its extract contains cytosterol and piperine which capable of affecting the reproductive hormones in male Siamese catfish. The purpose of study to evaluate the effect of java long pepper extract on spermatozoa motility of mustika common carp. Java long pepper extract was injected intramuscularly in the first of week with doses of 0.9 ml kg, 1.8 ml kg and 3.6 ml of body weight, respectively. Ovaprim in a dose of 0.3 ml body weight was administered as a positive control, whereas 0.1 ml of sodium solution was applied as a negative one. The result was observed in the second of week. Based on the ANOVA test showed that java long pepper extract has a significant effect on spermatozoa motility of mustika common carp (P<0.05). Further, according to the Tukey test that the highest spermatozoa motility rate was yield from a dose of 1.8 ml of java long pepper extract kg by body weight 69.5%.