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Background: Worm infection is one of the health problems in all countries, including Indonesia. The most common worm infection is infection caused by Soil Transmitted Helminth (STH) group. Primary school are the age group who are susceptible to infection with intestinal worms. Worm infections can have a serious impact if it is not treated with the administration of deworming drugs and a clean and healthy lifestyle of the person. Aims: This research was conducted with the aim of finding out risk factors such as the characteristics of children and parents, childrens behavior, and environmental sanitation related to the incidence of STH infection in children aged 6-12 years in Dusun II and IV of Manusak Village, Kupang Timur District, Kupang Regency in 2019. Method: This research uses observational analytic research with cross sectional design. The research subjects were 56 children. Data were collected using questionnaires then analyzed using Chi Square test (X2). Results: Worm examination is done by the Kato- Katz type indirect method. Based on the results of Kato-Katz examination, eggs of Ascaris lumbricoides were found in 54 children (96.4%). Conclusion: The Chi Square test results showed that the risk factor for STH infection was the habit of not washing hands with (P <0.05).