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Pretreatment before conducted plant extraction using Pulsed Electric Field (PEF) has been reported improve the yield and quality extracts. The specific input energy of PEF is an energy needed for each unit of volume of material during the PEF process which is expressed in units of kJ / cm3. It was influenced by voltage and duration of PEF. In this study, the relationship between specific input energy using variation voltage and duration of PEF was studied on extraction of areca seed. This research examined tannin levels and changes in areca cell structure before and after PEF. Areca (Areca catechu L.) is one of the plants that contain phenolic compounds as tannin as antioxidant. To obtain this compound, it was required the extraction process of areca seeds. This research used Factorial Randomized Block Design with two factors. The first factor was PEF voltage with three levels of 3, 4 and 5 kV and the second factor was PEF duration with three levels of 3, 5 and 7 minutes. The obtained data was analyzed by ANOVA. The input energy of PEF from 1.92-12.44 kJ/cm3 did not show significant effect on tannin content of areca seeds extract. However, when it compared with control (without PEF), better results was obtained on the tannin content. Scanning Electron Microscopic analysis from areca seed powder showed differences structure between control and PEF treatment. This study indicates that PEF pretreatment offers great promise in tannin extraction.