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Diabetes mellitus is a disease characterized by chronic hyperglycemic conditions. Hyperglycemia condition can increase free radicals in the body. Excessive free radicals will be toxic and cause oxidative stress. Oxidative stress can be characterized by high lipid peroxidation activity and reactive oxygen species (ROS). Free radical activity can be prevented with antioxidants. The materials that contain strong antioxidants are black soybeans tempe and purple sweet potatoes. The antioxidants are isoflavones and anthocyanins. This study aimed to determine the effect of black soybean Tempe and purple sweet potato extract on reactive oxygen species (ROS) activity in liver DMT2 mode. In this study, mice were made diabetic with a high-fat diet, 10% sucrose drink and injected with multiple low-dose streptozotocin. The animals experiment were divided into 7 groups: Negative control (K-), Positive Control (K+), Treatment 1 (P1), Treatment 2 (P2), treatment 3 (P3), Treatment 4 (P4), Treatment 5 ( P5). Measurement ROS using ELISA method. The content of antioxidants of black soybean tempe and purple sweet potato extract can lower blood glucose levels and affects ROS activity.