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Mining activities have a positive impact on the economy but have a negative impact on the environment due to the emergence of acid mine drainage through this activity. Conventional acid mine drainage treatment is an activity that requires high energy and costs, so it is necessary to study economic and sustainable acid mine drainage treatment processes. The development of acid mine drainage treatment involves the use of microalgae for remediation, or commonly referred to as phycoremediation. The obstacle in the application of mine acid water treatment is the characteristics of wastewater which limits the growth of microalgae, where acid mine drainage has a high metal content, and low pH. In this study, acid mine drainage with different concentration was used as a growth medium for Nannochloropsis sp. and growth kinetic of Nannochloropsis sp. is investigated, as well as the effect of metal exposure and pH variation on the development of Nannochloropsis sp.. The output of this study is expected to be able to provide an overview of the possible application of phycoremediation as a biological acid mine treatment process.