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The aims of this reseach were to determine the antibacterial activity of methanol extract from sambang darah (Excoecaria cochichinensis L.) leaves againts to the growth of the Staphylococcus epidermidis acne bacteria and to determine the most effective concentration of the tested samples. Antibacterial test was performed by variation concentrations of extract 2 mg/mL, 4 mg/mL, 6 mg/mL, 16 mg/mL, 32 mg/mL, and 64 mg/mL, antibiotic clindamycin as positive controls, and DMSO as negative control. The antibacterial activity of methanol extract from sambang darah (Excoecaria cochichinensis L.) leaves showed that the Staphylococcus epidermidis growth inhibitory was increased when the concentration of methanol extract from sambang darah (Excoecaria cochichinensis L.) leaves increased. A concentration of 64 mg/mL indicated the highest inhibition zone (12.94 mm). Based on Porce et al., (2003) the average of inhibition zone of 64 mg/mL concentration was summarized as moderate category that can inhibit the growth of Staphylococcus epidermidis bacteria.