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The majority of East Java people only use ferns as ornamental plants, even though ferns have potential in the medical field. The fern contains secondary metabolites so they are often called medicinal plants. Anatomical observations supported by histochemical tests can provide the type of secretory structure containing secondary metabolites and the content of secondary metabolites accumulated in these structures. This study began with exploration in the Tahura Soeryo area in June-July 2019. The results of the exploration were Pteris biaurita L., P. maricella V., P. quadriaurita Retz., P. linearis Poir, P. mertensiodes Wild , P. tripartita Sw., P. aspericaulis Wall. ex J. Agardh. , P. longipes D. Don, and P. linearis Poir. The rhizomes and fronds samples were cut across then tested with various types of reagents. Secondary metabolites were found in secretory tissues, vacuoles, and cytosol rhizomes and fronds of parenchymal cells in Pteris.