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Neural tube defects are congenital deformities that involve the covering of the central nervous system that starts during embryonic development when the neural tube fails to close completely. One of the most common types of birth defects which revealed a multifactorial in origin with contributions from both genetic and environmental factors. The main objective is to determine respondents profile, knowledge level on nature, prevention, and management of neural tube defects and its relationship. The researchers used purposive sampling method, and descriptive correlational design to assess the level of knowledge of pregnant women regarding prevention of NTDs. The researcher constructed questionnaire and was used after being pilot tested to gather data to identified respondents. Using frequency counts, percentages, weighted arithmetic mean and ranking the results showed that most of the respondents have an average level of knowledge on its nature, prevention, and management of NTDs. However, majority of which belongs to younger age (0.06432), low educational level (0.25756) and no maternal history of NTDs (0.25792) than older individual. Therefore, the researchers ruminate that it is necessary to teach pregnant women about NTDs, especially the factors that contribute to its occurrence. Health teaching is recommended for older respondents and to those who have a maternal history of NTDs. All clients of interest must be educated, regardless of educational status, as the result have showed that even highly educated respondents have less knowledge of the aforesaid congenital anomaly. Distributions of magazines, brochures and pamphlets are vital instruments to enhance their knowledge and to orient them about preventive measures to preserve the life of the mother and the child.