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Abstract Objective: To identify the effect of health education using video on mothers knowledge and attitudes about behavior problems among preschool children. Methods: This research was a quasi-experimental study with a pre-test-post-test control group design. The sampling technique was purposive sampling, and it was divided into an intervention group (n=61) and control group (n=61). The intervention group was given intervention using a two-dimensional animated video, while the control group used leaflets. Knowledge and attitudes of the mother were measured using a questionnaire. Data analysis used Wilcoxon, Mann Whitney test, Kruskal-Walli, and logistic regression. Results: There was a significant difference in knowledge (p=0.004) and attitude (p=0.04) of the intervention group (video) compared to the control group (leaflet) with a cohen value of knowledge (d=0.67) and attitude (d=0.55) that indicate a moderate size effect. Conclusion: Health education using video increase knowledge and attitudes of mothers about the behavior problem of preschool children.