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According to Ministry of Research, Technology and University, there are 750 million fresh graduate in every year. Survey from Monster.com in 2018 found29% fresh graduate new employee quit their first job in less than a year of joining, most of them because of under-prepared in their first job so it is difficult to adapt. This study aim to find out effect of work environment, orientation and experience of internship on successful adaptation of work for fresh graduate generation Y new employees. This study used a sample of 75 people, the questionnaire data was then processed using multiple linear regression tests which included t-tests and F-tests. The test results decided that the orientation period and internship experience had a positive effect on the adaptation of fresh graduate generation Y employees. While the work environment has no effect on the adaptation of Generation Y fresh graduate employees. Together (simultaneously) the three factors (work environment, orientation period, and internship experience) have a positive influence on the adaptation of Generation Y fresh graduate employees. From the results of 75 questionnaires also found 61 people (81.3%) need less than six months, eight people (10.7%) need six months, two people (2.7%) need 7-12 months, and four people ( 5.3%) requires more than 12 months from the first day of work to adapt.