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Maturation of Sheep Oocytes with Antioxidant α-Tocopherol which are activated by Parthenogenesis
Sri Gustina1, Hasbi Hasbi2, Iman Supriatna3, Mohamad Agus Setiadi3*

1Department of Animal Husbandry, Faculty of Animal Husbandry and Fisheries, Universitas Sulawesi Barat, Jl. Prof. Dr. Baharuddin Lopa, Tande Timur, Majene 91412, Indonesia
2Department of Animal Production, Faculty of Animal Science, Hasanuddin University, Jl. Perintis Kemerdekaan Km.10, Makassar 90245, Indonesia
3Division of Reproduction and Obstetrics, Department of Veterinary Clinic, Reproduction, and Pathology, Bogor Agricultural University, Jl. Agatis, Kampus IPB Darmaga, Bogor 16680, Indonesia
*E-mail: setiadi03[at]


This study was conducted to investigate the competence of sheep oocytes which are matured with antioxidant α-tocopherol by parthenogenesis activation. Oocytes from slaughterhouse were matured in tissue culture medium (TCM)-199 supplemented with 10% fetal bovine serum (FBS), 10 IU/ml pregnant mare serum gonadotrophin (PMSG), 10 IU/ml human chorionic gonadotrophin (hCG), 1 µg/ml estradiol, 50 µg/ml gentamycin, and with or without 150 µM α-tocopherol supplementation, then were parthenogenetically activated by exposing to 5 µM calcium ionophore for 5 min, and in medium containing 5 µg/ml cytochalasin B for 4 h. Furthermore oocytes were cultured in TCM 199 + 10% FBS either with or without supplementation of 150 μM α-tocopherol. Result of this experiment revealed that the percentages of activated oocytes with supplementation of 150 µM α-tocopherol only in maturation medium (63.41%) significantly higher (P<0.01) than the control group (50.00%) and the supplemented only in culture medium (51.02%). Furthermore, no significantly difference (P>0.05) in the number of oocytes with 1 pronucleus (PN), 2 PN, and more than 2 PN. It is concluded that the supplementation of 150 μM α-tocopherol on the maturation medium could also increase the percentage of activated oocytes optimally.

Keywords: Antioxidant α-tocopherol, in vitro maturation, ovine oocytes, parthenogenesis

Topic: Animal reproduction


Conference: The 2nd Iternational Conference of Animal Science and Technology (ICAST 2019)

Plain Format | Corresponding Author (Sri Gustina)

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