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Organic contaminant is one of the environmental issues in wastewater. Organic contaminants in wastewater may produce toxic chemicals during disinfection. Nowadays, food industries and restaurants are the main contributor of organic contaminant in wastewater. Organic contaminant caused the increase of BOD (Biological Oxygen Demand), COD (Chemical Oxygen Demand), TSS (Total Suspended Solid), and decrease DO (Dissolved Oxygen). The purpose of this article is to evaluate the efficiency of removing organic contaminant from wastewater with natural biofilter from banana tree. The efficiency of banana tree was analysed in terms of decreasing BOD, COD and the content of TSS. The parts of banana tree that can be removed organic contaminant from wastewater is its stem. Banana stem used as media natural biofilter because of its abundant availability in Indonesia. The disadvantages of banana tree was it can be easy placed by bacteria. Bacteria will develop well on banana stem because banana stem is moist enough and consist of much water in it. Banana stem contains cellulose fibers that can be used as media biofilter because the characteristics of it, such as abundantly available, biodegradable, renewable, cheaper, low abrasive nature, interesting specific properties, and exhibit good mechanical properties. However, the disadvantages of cellulose fibers were low thermal stability, moisture absorption, and poor compatibility.