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Basil (Ocimum sactum L.) contains saponins, flavonoids, polyphenols and tannins which elicit its antimicrobial activity. This research was true experimental laboratory with completely randomized design (CRD) with pre-posttest with control group. Freshly processed Basil leaves filtrate (Ocimum sactum L.) at concentrations of 40%, 50%, 60%, 70%, 80% were used as sample and the inhibitory activity against Escherichia coli ATCC 25922 was determined with 4 times of replication. Chloramphenicol oxoid disk was used as positive control. The average diameter of the inhibition zone of basil leaves filtrate against Escherichia coli ATCC 25922 at concentrations of 40%, 50%, 60%, 70%, 80% were 5.94 mm; 6.39 mm; 6.71 mm; 7.47 mm; and 7.94 mm, respectively. There was significant effect (P< 0,05) of basil leaves filtrate (Ocinum sanctum L) at various concentrations to the diameter of the inhibition zone against Escherichia coli ATCC 25922.