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This research was carried out to determine the phytochemical and antioxidant activity from the fruit of Scorodocarpus borneensis Becc. The partition of extract was prepared by fractination using gradient elution with hexane ethyl acetate, ethanol, methanol, and 70% methanol respectively and stirred for 24 hours. The crude fraction then concentrated using rotary evaporator at 400C. Qualitative determination by phytochemical screening were tested for the presence of flavonoids, alkaloids, tannins, phenols and terpenoids, while quantitative determination of total phenol and antioxidant activity by DPPH free radical scavenging activity was carried out using colorimetric methods. The yields of crude fraction were obtained 0,47% (hexane), 0,09% (ethyl acetate), 0,30% (ethanol), 1,23% (methanol) and 1,89% (70% methanol). Phytochemical screening of S. borneesis fruit crude fractions revealed the presence of all tested variable on ethanol, methanol and 70% methanolsolvent. The highest total phenolic content and antioxidant activity of S.borneensis fruit was obtained from fractionation with methanol solvent, that is 927,36 mgGAE/g and 14,54ppm respectively.