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Bioactive Components and Properties of Hexan Extract and Its Fractions from Kitolod (Isotoma longiflora (L) Presl.) Leaves
Sukardi*1, Ahmad Yunus2, Danar Praseptiangga3, and Setyaningrum Ariviani3

1Authors- Department of Food Technology, University of Muhammadiyah Malang, INDONESIA.
(E-mail: sukardiitp[at]gmail.com)

2Author-s Department of Agrotechnology, Sebelas Maret University, INDONESIA.
(E-mail: yunus.uns7[at]yahoo.com)

3Author-s Department of Food Science and Technology, Sebelas Maret University, INDONESIA.
(E-mail: praseptiangga_de[at]yahoo.com, setya_ariviani[at]yahoo.com)


Abstract

Technological advances improve the human life welfare, but it also raises environmental problems such as cigarette smoke, ozone damage, air and water pollution that cause free radicals. Continuously, the results of free radicals encourage the oxidative stress that defines as the imbalance between the productions of reactive oxygen species (ROS) with antioxidants to repair damage. This condition can stimulate the interference to all cellular components, including protein, lipids, carbohydrates, acids and nucleates, as the result it will affect physiological conditions (aging and exercise) and even diseases (inflammation, diabetes, cardiovascular and neurodegenerative, and cancer.) To improve human health and prevent several problems due to free radicals is needed antioxidants from food and secondary metabolites from plants. Antioxidant can interact safely with free radicals, which could terminate the reaction before the molecule interference. Kitolod (Isotoma longiflora (L) Presl) mostly known as traditional medicine plant that contains secondary metabolites. According to previous research, it can treat several disease such as bronchitis, asthma, anticancer, anti-inflammatory, and analgesic (Hariana, 2008; Hapsari et al., 2016). This study aimed to obtain bioactive components from hexane extracts, and their fractions from the leaves of kitolod (Isotoma longiflora (L) Presl). The procedure started, chitolod leaves were initially dried at 50oC then passed 40 mesh sieving. Kitolod leaves powder were extracted with hexane. Then followed by the chromatography column with hexane-fractionated extract: ethyl acetate eluent. LCMS was applied for bioactive compounds identification from fractions. Bioactive activity evaluation included antioxidant activity, anti-inflammatory then inhibition of alpha amylase enzyme and glucosidase. The results showed that fractionation of hexane extract was obtained in 8 fractions. Antioxidant activity with DPPH in fractions 1 to 8 (IC 50) ranges from 8, 6 - 74, 5 µg / ml. The anti-inflammatory activity of the fraction at a concentration of 100 µg / ml ranged from 1.4 - 42.5 percent, whereas for the inhibitory activity of the -amylase enzyme was from 13.6 to 74.0 percent and -glucosidase was 29 - 55 percent (Table 1). Bioactive compounds in the 8th fraction were identified: benzoic acid, Sabinene, Myrcene, Thymol, Nojigiku alcohol, Capillin, β Caryophyllene, Cis Spiroketalenolether, polyyne, Nerolidol, Chrysandiol, Clovane 2 β, 9 α diol, Santilline, β Caryophyllene, Cis Spiroketalenolether, polyyne, Nerolidol, Chrysandiol, Clovane 2 β, 9 α diol, Santamarine, β Caryophyllene, Cis Spiroketalenolether, polyyne, Nerolidol, Chrysandiol, Clovane 2 β, 9 α diol, Santamarine, β Caryophyllene, Cis Spiroketalenolether, polyyne, Nerolidol, Chrysandiol, Clovane 2 β, 9 α diol, Santamarine, CICICOLIC CIC Cikanol F, β Sitosterol (Table 2). The results indicated that from 8 fractions there was a significantly diffe

Keywords: bioactive, hexane extract, fraction, antioxidants, anti-inflammatory, antidiabetic

Topic: Environmentally Sustainable Agriculture

Link: https://ifory.id/abstract/4EXdG7UyTtfL

Conference: International Conference on Bioenergy and Environmentally Sustainable Agriculture Technology (ICONBEAT 2019)

Plain Format | Corresponding Author (Sukardi Sukardi)

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