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Antibacterial Activity of Crude Extract of Endophytic Fungi Aspergillus sp. Against Methicillin Resistant Staphylococcus aureus (MRSA)
Ni Ketut Yuliana Sari (a*), Retno Kawuri (b), Ni Made Susun Parwanayoni (b)

(a) Postgraduate School Universitas Airlangga
Jalan Airlangga No. 4-6, Airlangga, Gubeng, Surabaya, Jawa Timur 60115, Indonesia
*ni.ketut.yuliana-2018[at]pasca.unair.ac.id

(b) Department of Biology, Faculty of Science, Udayana University, Bali
Jalan Kampus Bukit Jimbaran 80361 Bali, Indonesia


Abstract

Antimicrobial resistance has become a public health problem in the world, one of which is Methicillin Resistant Staphylococcus aureus (MRSA). One way to overcome this problem is to find new antibacterial compounds with the use of endophytic fungi. The purpose of this study was to test the antibacterial activity of crude extract of endophytic fungi Aspergillus sp. against MRSA and its contents in the secondary metabolites produced. Endophytic fungi Aspergillus sp. isolated from elephant ginger rhizome (Zingiber officinale var. Roscoe). This research is an exploratory research which is continued with experimental. The antagonist test of endophytic fungi Aspergillus sp. against MRSA produces a inhibition zone of 32 mm. The inhibitory of crude extract of endophytic fungi Aspergillus sp. in the water phase is 13 mm and an ethyl acetate phase is 9 mm. Phytochemical analysis of crude extract of endophytic fungi Aspergillus sp. in the water phase shows the presence of group compounds alkaloids, flavonoids, saponins, and terpenoids. GCMS analysis shows the content of glycidyl oleate (28,44%); didodecyl phthalate (26,67%); cis-13-Octadecenoic acid, methyl ester (20,73%); oleic acid, 3-hydroxypropyl ester (12,98%); 5Hydroxymethylfurfural (4,04%); benzophenone (2,42%); Propanamide, N-ethyl- (2,09%); benzene, nitro- (1,33%) and azulene (1,31%).

Keywords: Antibacterial; Endophytic fungi; Aspergillus sp.; Methicillin Resistant Staphylococcus aureus

Topic: Health and Medicine

Link: https://ifory.id/abstract/4H8mZUWNRArc

Conference: International Conference Postgraduate School Universitas Airlangga (ICPS 2019)

Plain Format | Corresponding Author (Ni Ketut Yuliana Sari)

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