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This study aims to obtain an innovative on general chemistry lab guide for agrotechnology study programs. The research was a development research referring to the ADDIE model (Analysis, Design, Development, Implementation, and Evaluation). Practical guidebooks were arranged following the steps of the learning model based on Problem Based Learning. Research locations in the Chemistry Laboratory, Faculty of Agriculture, Agrotechnology Study Program, Medan Area University. The results showed that the validation of basic chemistry practicum guidebooks that had been developed in general showed an average value of 3.90 meaning very valid and did not need to be revised and was very feasible to use. The learning outcomes of the practitioner have an N-gain average of 0.65 was categorized as medium. The results of the practical skills average grade value of 93.52 which is categorized very well. The results of the practical guideline assessment conducted by the practitioner after using a chemical practicum guidebook that has been developed has a percentage of 88.10 percentage with a very good category.