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Background: Continous surveillance of DENV tranmission pattern is undoubtedly important to provide proper input to the dengue prevention and control programme. However, the DENV transmission pattern in outbreak event is still under research. This information is crucial for dengue outbreak preparedness, particularly in dengue endemic area Purpose: This study interested to analyse the spatio temporal distribution of dengue cases during outbreak event in the area of study Methods This is cross sectional study which conducted in Banyumas Regency, Central Java Indonesia. 404 of dengue cases coordinates during 2016 outbreak in Banyumas Regency, Indonesia were collected. Data was analysis using overlay technique spatial analysis, consist of analysis of point pattern method, Nearest Neighbour Index, convex hulls and cluster analysis of illness date with SatScan software. Results: Two clusters area were identified during outbreak, with the initial place of outbreak is surprisingly occured in the border area, assuming that human movement and imported cases might play role in dengue outbreak event. Based Nearest Neighbour Index analysis, distribution pattern of outbreak in the study area is clustered. This could be as consequence of limited flight range of Aedes sp mosquito, high density population and breeding site of mosquitoes. Conclusion: Information from our study highlighted where and when public health resources should be concentrated to control dengue transmission. Our study also suggested that besides the hotspot area, we need to aware other areas with high risk of imported cases such as border area.