Indonesia Conference Directory

Bandotan (Ageratum conyzoides L) herbaceous plants have been empirically used for wound medicine and digestive disorders. The treatment is related to its pharmacological activity as an antibacterial. The purpose of this study was to isolate responsible compounds as antibacterial. The research phase is extraction using maceration method, fractionation by liquid vacuum column chromatography followed by TLC, the same profile of TLC combined and then tested for its activity against Esceresia coli and Staphylococus aureus. The results of the antibacterial activity test showed that the inhibition of ethyl acetate extract was 14 mm, the combined fraction 4 was 16 mm and subfraction Fg4.2 was 14.5 mm at S aureus, whereas in E coli extract the ethylacetate extract was 11 mm, the combined fraction 4 was 13 mm and sub Fg4.2 of 10 mm. The minimum inhibition of combined Fg1.2 subfraction against S. aureus is 12,5 mg / ml and 25 mg / ml for E. coli. Identification results with UV-vis, FT-IR and GC-MS showed that in bandotan herbs contain compounds called coumarin, kromen, fatty acids and eicosan.