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Aceh-s patchouli is one of the superior local commodities, and its growth and yield could be improved in various means. One way to increase the patchouli production can be done by using bio-fertilizer mycorrhizal local strain. The purpose of this research was to study effect of bio-fertilizer mycorrhizal local specific strain on the growth and yield of patchouli in Entisols. The research was arranged by a non-factorial randomized block design with three replications. The investigated factor was the bio-fertilizer mycorrhizal from local specific strain with four levels, i.e. M0 (without mycorrhizae), M1 (Glomus mosseae), M2 (Gigaspora sp) and M3 (Mixing between Glomus mosseae and Gigaspora sp). The variety of patchouli used in this study was the Tapak Tuan variety. The observed parameters were plant height and stem diameter of patchouli at 45, 60 days after planting; additionally the parameter of patchouli yields consist of number of leaves, number of branches and weight of leaves were observed at 90 days after planting, and root colonization by mycorrhizae. The results showed that mixed mycorrhizal strains between Glomus mosseae + Gigaspora sp contributed to the best marks for increasing growth and yield of patchouli in Entisols. The similar trend result was also found in the root colonization by mycorrhizae. The strain of mycorrhizal of Gigaspora sp application was not effective for increasing the growth and yield of Aceh-s patchouli in Entisols.