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Knowledge of the structure of the human body at a macro level to the molecular level is fundamental to understanding bodily functions and how the structure and function of body organs change due to disease. An appropriate instrument is needed to measure the level of student knowledge about anatomy, especially knowledge about the structure and topography of organs.The purpose of this study is to find out which questions are valid and reliable to measure the level of knowledge in medical students regarding organs structure and topography.This research method was a survey approach, where 88 samples were collected randomly. Validity of the questions were analyzed by Pearson-s correlation coefficient, while Kappa-s coefficient agreement was chosen to analyze the reliability of questions. The result showed that there was 9 organ systems (dermatomuscular, endocrine, neurology, genitourinary, reproductive, special senses, cardiovascular, genitourinary and respiratory) that had analyzed, consisted of structure and topography questions. Most of the questions had valid and reliable criteria according to statistical analysis above. In conclusion, the instrument was valid and reliable to determine the level of knowledge pertaining organs structure and topography. Any additional questions should be analyzed, whether they had criterion related validity and reliability.