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The research is designed to identify the IGF-1 gene polymorphism association with litter size of Etawa Grade goats. This research used 48 Etawa Grade female goat bloods samples that has given birth more than once taken from Polewali Mandar regency of West Sulawesi province. Blood samples was collected via the jugular vein in vacutainer tubes containing EDTA anticoagulant to prevent blood clots and followed by DNA extraction. Then, the genetic variation of this gene candidates identified using Polymerase Chain Reaction - Restriction Fragment Length Polymorphism (PCR-RFLP) technique. IGF-1 gene polymorphisms were detected by cutting amplimer using restriction enzyme Haelll. The research results indicate that gene polymorphism is found in exon 4 gene IGF-1. This is the transition GG/CC in which two alleles in the population found. The frequency of allele A is 0,72, while allele B is 0,28. The results of Hardy-Weinberg equilibrium for each region using the chi-square formula is 0,248 which is in Hardy-Weinberg equilibrium (P>0.05). Value of heterazygocity (He) is 0,409 which indicates that the value of genetic diversity of PE goats is low. IGF-1 genotype of Polimorphism / Haelll has a significant effect on the litter size (P<0.01), Average litter size for each genotype was different (AA genotipe = 1,95 ± 0,55, AB = 1,73 ± 0,73 and BB = 1,67 ± 0,29). This shows that there is difference in litter size for each types of genotype and AA genotype tends to have higher litter size. Allele A has higher litter size effect compares to allele B. Therefore, IGF-1/Haelll genotypes can be used as a genetic marker for litter size selection.