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Objective: This study aimed to compare the prevalence of Pseudomonas aeruginosa infection and the phenotypic and genotypic resistance characteristic to cephalosporin antibiotic between men and women at dr. Wahidin Sudirohusodo Hospital in Makassar, Indonesia. Method : Between January to July 2019, a total of 50 Pseudomonas aeruginosa isolates were obtained from patients suffering from various types of infections, 17 of which (34%) were women and 33 (66%) were men. These isolates were tested for antibiotic sensitivity by Vitek-2, extracted for DNA and amplified by multiplex polymerase chain reaction to detect SHV,TEM and CTX-M extended spectrum beta-lactamase genes. Results: The 17 isolates from women were pus (10), sputum (2), urine (2), blood (2) and pleura aspirate (1). The isolates phenotype sensitive to ceftazidime antibiotic were 14 samples (82.3%), 2 (12.5%) were resistant, and 1 was intermediate (6.25%). The ESBL genes detected were TEM 13 (76.5%) isolates, SHV 1 (5%) isolate, CTX-M 9 (52%) isolates. Meanwhile in men the isolates were from pus (12), sputum (14), blood (2), bronchial washing (2), tissue (2), ear secretion (1) and gastric lavage (1). There were 27 isolates (81.8%) sensitive to ceftazidime antibiotics, 5 (15.1%) were resistant, and 1 intermediate (3.03%). The ESBL genes that were detected in isolates from men were TEM 27 (81.8%) isolates, SHV 2 (6%) isolates, CTX-M 17 (51%) isolates. Conclusion: Our study showed that there was a similar low prevalence of phenotype among resistance various types of infection in both men and women. TEM and CTX-M were the predominantly detected genes, and SHV was the least found among, the Pseudomonas aeruginosa isolated from either women and men patients. This indicated the potential of the isolates to become resistant.