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Semarang city is one of the areas in Central Java Province with increasing number of DHF cases from 2011-2015. The aim of this was to describe the dengue virus strain and host characteristics of Dengue Hemorrhagic Fever (DHF)-s suspect on three health facilities in Semarang City. This cross sectional design used variables of virus strain, patient characteristic, blood type, domicile, and the result of diagnose. The samples were 67 respondents with certain criteria. The number of 21 respondents who were detected DHF examined by PCR to know dengue virus strain. The data was analyzed using frequency distribution and bivariate analysis. The result showed that respondents who were diagnosed with DHF were mostly male 34.3%; age range around children 35.3%; blood type O, 8 out of 21 respondents and the dengue virus stereotype that was found was DEN-2, DEN-3, DEN-4.