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Abstract Objective: This study aims to investigate phosphate content which dissolves from tooth enamel after soaked with brown algae Padina sp extract. Method: Research type used is experimental research with pre and post-test group control design. Sample using maxillary first premolar that has separated crown and roots then planted with acrylic resin. Phosphate level test performed by an ascorbic acid method and then read the absorbance on UV-vis spectrophotometer. Result: Mean of value on Padina extract has no significant change (p>0.05), and positive control show significantly different (p<0.05). Conclusion: Brown algae Padina sp. more likely to be a material that inhibits the demineralization process so that this material is more potential as an applicative material